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random blood glucose 16 7 mmol l rhadamts13  (R&D Systems)


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    R&D Systems random blood glucose 16 7 mmol l rhadamts13
    ADAMTS13 maintained renal function and attenuated renal fibrosis in vivo . Control mice were treated with vehicle (Control). The STZ-treated mice were infused with vehicle (DN) or <t>rhADAMTS13</t> (DN + rhADAMTS13). rhADAMTS13 (2.6 ug/kg body weight) was injected into the tail vein daily for the subsequent 7 d. (A) The workflow of animal experiments. (B) Representative renal immunohistochemical staining of ADAMTS13 in the kidney. Scale Bar: 30 μm. (C) Representative renal morphological images of mice were shown. Hematoxylin-Eosin (HE), Masson’s trichrome and Periodic Acid-Schiff (PAS) staining of kidney slices. Scale Bar: 30 μm. (D) Body weight. (E) Serum glucose. (F) BUN. (G) Scr. (H) Urinary volume. (I) Proteinuria. (J) Urinary KIM-1. (K) Renal KIM-1 mRNA expression. ADAMTS13: a disintegrin and metalloprotease with a thrombospondin type 1 motif member 13; DN: diabetic nephropathy; BUN: blood urea nitrogen; Scr: serum creatinine; KIM-1: kidney injury molecule-1. Results were presented as mean ± SEM. N = 5, ** P < 0.01, *** P < 0.001, one-way ANOVA followed by Tukey’s post hoc test.
    Random Blood Glucose 16 7 Mmol L Rhadamts13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+adamts13/Recombinant+Human+ADAMTS13+Protein%2C+CF/pmc13037144-108-11-18
    Average 94 stars, based on 35 article reviews
    random blood glucose 16 7 mmol l rhadamts13 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "ADAMTS13 ameliorates diabetic nephropathy by Nrf2/GPX4/eNOS signaling pathway"

    Article Title: ADAMTS13 ameliorates diabetic nephropathy by Nrf2/GPX4/eNOS signaling pathway

    Journal: Renal Failure

    doi: 10.1080/0886022X.2026.2646089

    ADAMTS13 maintained renal function and attenuated renal fibrosis in vivo . Control mice were treated with vehicle (Control). The STZ-treated mice were infused with vehicle (DN) or rhADAMTS13 (DN + rhADAMTS13). rhADAMTS13 (2.6 ug/kg body weight) was injected into the tail vein daily for the subsequent 7 d. (A) The workflow of animal experiments. (B) Representative renal immunohistochemical staining of ADAMTS13 in the kidney. Scale Bar: 30 μm. (C) Representative renal morphological images of mice were shown. Hematoxylin-Eosin (HE), Masson’s trichrome and Periodic Acid-Schiff (PAS) staining of kidney slices. Scale Bar: 30 μm. (D) Body weight. (E) Serum glucose. (F) BUN. (G) Scr. (H) Urinary volume. (I) Proteinuria. (J) Urinary KIM-1. (K) Renal KIM-1 mRNA expression. ADAMTS13: a disintegrin and metalloprotease with a thrombospondin type 1 motif member 13; DN: diabetic nephropathy; BUN: blood urea nitrogen; Scr: serum creatinine; KIM-1: kidney injury molecule-1. Results were presented as mean ± SEM. N = 5, ** P < 0.01, *** P < 0.001, one-way ANOVA followed by Tukey’s post hoc test.
    Figure Legend Snippet: ADAMTS13 maintained renal function and attenuated renal fibrosis in vivo . Control mice were treated with vehicle (Control). The STZ-treated mice were infused with vehicle (DN) or rhADAMTS13 (DN + rhADAMTS13). rhADAMTS13 (2.6 ug/kg body weight) was injected into the tail vein daily for the subsequent 7 d. (A) The workflow of animal experiments. (B) Representative renal immunohistochemical staining of ADAMTS13 in the kidney. Scale Bar: 30 μm. (C) Representative renal morphological images of mice were shown. Hematoxylin-Eosin (HE), Masson’s trichrome and Periodic Acid-Schiff (PAS) staining of kidney slices. Scale Bar: 30 μm. (D) Body weight. (E) Serum glucose. (F) BUN. (G) Scr. (H) Urinary volume. (I) Proteinuria. (J) Urinary KIM-1. (K) Renal KIM-1 mRNA expression. ADAMTS13: a disintegrin and metalloprotease with a thrombospondin type 1 motif member 13; DN: diabetic nephropathy; BUN: blood urea nitrogen; Scr: serum creatinine; KIM-1: kidney injury molecule-1. Results were presented as mean ± SEM. N = 5, ** P < 0.01, *** P < 0.001, one-way ANOVA followed by Tukey’s post hoc test.

    Techniques Used: In Vivo, Control, Injection, Immunohistochemical staining, Staining, Expressing

    Related Articles

    Recombinant:

    Article Title: Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
    Article Snippet: .. Briefly, flat-bottom 96-well microtiter plates (Thermo Fisher Scientific, 167,008) were coated with Recombinant Human ADAMTS13 (Full Length) Protein (R&D Systems, 6156-AD-020) at 50 ng/well, incubated overnight at 4°C, and blocked with ELISA blocking buffer (1 × phosphate-buffered saline (PBS), 2% bovine serum albumin (BSA), 0.05% NaN 3 ) at room temperature for 1 h. Three-fold serial dilutions of the mAbs from 1 μg/mL to 0.001 μg/mL in ELISA diluent (1 × PBS, 1% BSA, 0.05% NaN 3 ) were incubated in triplicate on the protein-coated wells for 1 ~ 2 h. Chimeric A10 and mA10 were used as positive controls, and 8A7 and 16E8, and mouse IgG2b-UNLB (SouthernBiotech, 1090–01) were used as negative controls for human and mouse ELISA tests, respectively. .. After washing the ELISA plates, human or mouse mAbs bound to the coated ADAMTS13 protein were detected by Goat Anti-Human IgG-AP (SouthernBiotech, 2040–04) or Goat Anti-Mouse IgG-AP (SouthernBiotech, 1030–04) with an alkaline phosphatase substrate (Sigma-Aldrich, S0942), respectively.

    Article Title: ADAMTS-13-regulated nuclear factor E2-related factor 2 signaling inhibits ferroptosis to ameliorate cisplatin-induced acute kidney injuy.
    Article Snippet: CP was purchased from MCE. .. Recombinant human ADAMTS13 (rhADAMTS13) were purchased from R&D Systems (Minneapolis, MN). .. BUN (C013-2, Nanjing Jiancheng Bioengineering Institute, China) and Scr (ab65340, Abcam) levels in serum were measured using the corresponding detection kits in accordance with the manufacturer’s instructions.

    Article Title: A Fluorescence Cross-Correlation-Spectroscopy-Based Immunoassay for Rapid, Selective, and Accurate Protein Sizing in Human Plasma, Applied to the von Willebrand Factor.
    Article Snippet: Multimeric abnormalities in plasma von Willebrand factor (VWF) cause bleeding or clotting disorders.. Electrophoretic analysis of multimers is used to detect such abnormalities but is qualitative, slow, and difficult to standardize.. Fluorescence correlation spectroscopy (FCS) is a good alternative but is affected by low selectivity and concentration bias.

    Article Title: Recombinant human ADAMTS13 attenuates LPS-induced acute kidney injury and renal microangiopathy in murine advanced liver fibrosis by cleaving vWF.
    Article Snippet: .. Recombinant Human ADAMTS13 (Full Length) Protein, Carrier-free used as rhADAMTS13 was obtained from R&D systems (Minneapolis, MN, USA). ..

    Article Title: Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
    Article Snippet: Chimeric A10 and mA10 were used as positive controls, and 8A7 and 16E8, and mouse IgG2b-UNLB (SouthernBiotech, 1090–01) were used as negative controls for SPR analyses of human and mouse antibodies, respectively. .. The binding curves were obtained by injecting 2-fold serial dilutions of Recombinant Human ADAMTS13 (Full Length) Protein (R&D Systems, 6156-AD-020) ranging from 80 nM to 0.625 nM in PBS containing 0.05% Tween 20 (PBS-T) (Supplemental Fig. and ). .. The operation parameters were as follows: temperature, 25 °C; flow rate, 30 μL/min; contact time, 240 s; dissociation time, 900 s. The obtained binding curves of each antibody were analyzed using 1:1 binding model in Biacore T200 Evaluation Software version 2.0 (Cytiva).

    Article Title: ADAMTS-13-regulated nuclear factor E2-related factor 2 signaling inhibits ferroptosis to ameliorate cisplatin-induced acute kidney injuy
    Article Snippet: CP was purchased from MCE. .. Recombinant human ADAMTS13 (rhADAMTS13) were purchased from R&D Systems (Minneapolis, MN). .. BUN (C013-2, Nanjing Jiancheng Bioengineering Institute, China) and Scr (ab65340, Abcam) levels in serum were measured using the corresponding detection kits in accordance with the manufacturer’s instructions.

    Article Title: ADAMTS13 inhibits H<sub>2</sub>O<sub>2</sub>-induced human venous endothelial cell injury to attenuate deep-vein thrombosis by blocking the p38/ERK signaling pathway
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) that provided by BeNa Culture Collection (Henan, China) were incubated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) which was decorated with 10% fetal bovine serum (FBS; GE Healthcare Life Sciences) and 1% penicillin-streptomycin (Beyotime, Shanghai, China) at 37°C with 5% CO2. .. To establish oxidative stress injury model in vitro, HUVECs were exposed to 0.5 mM H2O2 (Sigma-Aldrich, Merck KGaA) for 24 h. [21] To evaluate the effect of ADAMTS13 on HUVEC injury exposed to H2O2, the HUVECs were pretreated with 1, 4, or 8 nM recombinant human ADAMTS13 (rADAMTS13; rA13; R&D systems, Minneapolis, MN, USA) for 30 min prior to H2O2 treatment. .. [22,23] To figure out the mechanism of p38/ERK signaling pathway, p79350 (p38 agonist; Invitrogen, Carlsbad, CA, USA) was used to pre-treat HUVECs for 1 h and then treated with or without rA13 at concentrations of 1, 4, or 8 nM for 30 min before H2O2 treatment.

    Incubation:

    Article Title: Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
    Article Snippet: .. Briefly, flat-bottom 96-well microtiter plates (Thermo Fisher Scientific, 167,008) were coated with Recombinant Human ADAMTS13 (Full Length) Protein (R&D Systems, 6156-AD-020) at 50 ng/well, incubated overnight at 4°C, and blocked with ELISA blocking buffer (1 × phosphate-buffered saline (PBS), 2% bovine serum albumin (BSA), 0.05% NaN 3 ) at room temperature for 1 h. Three-fold serial dilutions of the mAbs from 1 μg/mL to 0.001 μg/mL in ELISA diluent (1 × PBS, 1% BSA, 0.05% NaN 3 ) were incubated in triplicate on the protein-coated wells for 1 ~ 2 h. Chimeric A10 and mA10 were used as positive controls, and 8A7 and 16E8, and mouse IgG2b-UNLB (SouthernBiotech, 1090–01) were used as negative controls for human and mouse ELISA tests, respectively. .. After washing the ELISA plates, human or mouse mAbs bound to the coated ADAMTS13 protein were detected by Goat Anti-Human IgG-AP (SouthernBiotech, 2040–04) or Goat Anti-Mouse IgG-AP (SouthernBiotech, 1030–04) with an alkaline phosphatase substrate (Sigma-Aldrich, S0942), respectively.

    Article Title: A Fluorescence Cross-Correlation-Spectroscopy-Based Immunoassay for Rapid, Selective, and Accurate Protein Sizing in Human Plasma, Applied to the von Willebrand Factor.
    Article Snippet: Multimeric abnormalities in plasma von Willebrand factor (VWF) cause bleeding or clotting disorders.. Electrophoretic analysis of multimers is used to detect such abnormalities but is qualitative, slow, and difficult to standardize.. Fluorescence correlation spectroscopy (FCS) is a good alternative but is affected by low selectivity and concentration bias.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
    Article Snippet: .. Briefly, flat-bottom 96-well microtiter plates (Thermo Fisher Scientific, 167,008) were coated with Recombinant Human ADAMTS13 (Full Length) Protein (R&D Systems, 6156-AD-020) at 50 ng/well, incubated overnight at 4°C, and blocked with ELISA blocking buffer (1 × phosphate-buffered saline (PBS), 2% bovine serum albumin (BSA), 0.05% NaN 3 ) at room temperature for 1 h. Three-fold serial dilutions of the mAbs from 1 μg/mL to 0.001 μg/mL in ELISA diluent (1 × PBS, 1% BSA, 0.05% NaN 3 ) were incubated in triplicate on the protein-coated wells for 1 ~ 2 h. Chimeric A10 and mA10 were used as positive controls, and 8A7 and 16E8, and mouse IgG2b-UNLB (SouthernBiotech, 1090–01) were used as negative controls for human and mouse ELISA tests, respectively. .. After washing the ELISA plates, human or mouse mAbs bound to the coated ADAMTS13 protein were detected by Goat Anti-Human IgG-AP (SouthernBiotech, 2040–04) or Goat Anti-Mouse IgG-AP (SouthernBiotech, 1030–04) with an alkaline phosphatase substrate (Sigma-Aldrich, S0942), respectively.

    Blocking Assay:

    Article Title: Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
    Article Snippet: .. Briefly, flat-bottom 96-well microtiter plates (Thermo Fisher Scientific, 167,008) were coated with Recombinant Human ADAMTS13 (Full Length) Protein (R&D Systems, 6156-AD-020) at 50 ng/well, incubated overnight at 4°C, and blocked with ELISA blocking buffer (1 × phosphate-buffered saline (PBS), 2% bovine serum albumin (BSA), 0.05% NaN 3 ) at room temperature for 1 h. Three-fold serial dilutions of the mAbs from 1 μg/mL to 0.001 μg/mL in ELISA diluent (1 × PBS, 1% BSA, 0.05% NaN 3 ) were incubated in triplicate on the protein-coated wells for 1 ~ 2 h. Chimeric A10 and mA10 were used as positive controls, and 8A7 and 16E8, and mouse IgG2b-UNLB (SouthernBiotech, 1090–01) were used as negative controls for human and mouse ELISA tests, respectively. .. After washing the ELISA plates, human or mouse mAbs bound to the coated ADAMTS13 protein were detected by Goat Anti-Human IgG-AP (SouthernBiotech, 2040–04) or Goat Anti-Mouse IgG-AP (SouthernBiotech, 1030–04) with an alkaline phosphatase substrate (Sigma-Aldrich, S0942), respectively.

    Saline:

    Article Title: Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
    Article Snippet: .. Briefly, flat-bottom 96-well microtiter plates (Thermo Fisher Scientific, 167,008) were coated with Recombinant Human ADAMTS13 (Full Length) Protein (R&D Systems, 6156-AD-020) at 50 ng/well, incubated overnight at 4°C, and blocked with ELISA blocking buffer (1 × phosphate-buffered saline (PBS), 2% bovine serum albumin (BSA), 0.05% NaN 3 ) at room temperature for 1 h. Three-fold serial dilutions of the mAbs from 1 μg/mL to 0.001 μg/mL in ELISA diluent (1 × PBS, 1% BSA, 0.05% NaN 3 ) were incubated in triplicate on the protein-coated wells for 1 ~ 2 h. Chimeric A10 and mA10 were used as positive controls, and 8A7 and 16E8, and mouse IgG2b-UNLB (SouthernBiotech, 1090–01) were used as negative controls for human and mouse ELISA tests, respectively. .. After washing the ELISA plates, human or mouse mAbs bound to the coated ADAMTS13 protein were detected by Goat Anti-Human IgG-AP (SouthernBiotech, 2040–04) or Goat Anti-Mouse IgG-AP (SouthernBiotech, 1030–04) with an alkaline phosphatase substrate (Sigma-Aldrich, S0942), respectively.

    Concentration Assay:

    Article Title: A Fluorescence Cross-Correlation-Spectroscopy-Based Immunoassay for Rapid, Selective, and Accurate Protein Sizing in Human Plasma, Applied to the von Willebrand Factor.
    Article Snippet: Multimeric abnormalities in plasma von Willebrand factor (VWF) cause bleeding or clotting disorders.. Electrophoretic analysis of multimers is used to detect such abnormalities but is qualitative, slow, and difficult to standardize.. Fluorescence correlation spectroscopy (FCS) is a good alternative but is affected by low selectivity and concentration bias.

    Binding Assay:

    Article Title: Optimization of anti-ADAMTS13 antibodies for the treatment of ADAMTS13-related bleeding disorder in patients receiving circulatory assist device support
    Article Snippet: Chimeric A10 and mA10 were used as positive controls, and 8A7 and 16E8, and mouse IgG2b-UNLB (SouthernBiotech, 1090–01) were used as negative controls for SPR analyses of human and mouse antibodies, respectively. .. The binding curves were obtained by injecting 2-fold serial dilutions of Recombinant Human ADAMTS13 (Full Length) Protein (R&D Systems, 6156-AD-020) ranging from 80 nM to 0.625 nM in PBS containing 0.05% Tween 20 (PBS-T) (Supplemental Fig. and ). .. The operation parameters were as follows: temperature, 25 °C; flow rate, 30 μL/min; contact time, 240 s; dissociation time, 900 s. The obtained binding curves of each antibody were analyzed using 1:1 binding model in Biacore T200 Evaluation Software version 2.0 (Cytiva).

    other:

    Article Title: Involvement of the ADAMTS13–von Willebrand factor axis in acute kidney injury in mice with liver cirrhosis
    Article Snippet: Lipopolysaccharide (LPS) derived from Klebsiella pneumoniae was purchased from Sigma-Aldrich (St. Louis,

    In Vitro:

    Article Title: ADAMTS13 inhibits H<sub>2</sub>O<sub>2</sub>-induced human venous endothelial cell injury to attenuate deep-vein thrombosis by blocking the p38/ERK signaling pathway
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) that provided by BeNa Culture Collection (Henan, China) were incubated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) which was decorated with 10% fetal bovine serum (FBS; GE Healthcare Life Sciences) and 1% penicillin-streptomycin (Beyotime, Shanghai, China) at 37°C with 5% CO2. .. To establish oxidative stress injury model in vitro, HUVECs were exposed to 0.5 mM H2O2 (Sigma-Aldrich, Merck KGaA) for 24 h. [21] To evaluate the effect of ADAMTS13 on HUVEC injury exposed to H2O2, the HUVECs were pretreated with 1, 4, or 8 nM recombinant human ADAMTS13 (rADAMTS13; rA13; R&D systems, Minneapolis, MN, USA) for 30 min prior to H2O2 treatment. .. [22,23] To figure out the mechanism of p38/ERK signaling pathway, p79350 (p38 agonist; Invitrogen, Carlsbad, CA, USA) was used to pre-treat HUVECs for 1 h and then treated with or without rA13 at concentrations of 1, 4, or 8 nM for 30 min before H2O2 treatment.



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    ADAMTS13 maintained renal function and attenuated renal fibrosis in vivo . Control mice were treated with vehicle (Control). The STZ-treated mice were infused with vehicle (DN) or rhADAMTS13 (DN + rhADAMTS13). rhADAMTS13 (2.6 ug/kg body weight) was injected into the tail vein daily for the subsequent 7 d. (A) The workflow of animal experiments. (B) Representative renal immunohistochemical staining of ADAMTS13 in the kidney. Scale Bar: 30 μm. (C) Representative renal morphological images of mice were shown. Hematoxylin-Eosin (HE), Masson’s trichrome and Periodic Acid-Schiff (PAS) staining of kidney slices. Scale Bar: 30 μm. (D) Body weight. (E) Serum glucose. (F) BUN. (G) Scr. (H) Urinary volume. (I) Proteinuria. (J) Urinary KIM-1. (K) Renal KIM-1 mRNA expression. ADAMTS13: a disintegrin and metalloprotease with a thrombospondin type 1 motif member 13; DN: diabetic nephropathy; BUN: blood urea nitrogen; Scr: serum creatinine; KIM-1: kidney injury molecule-1. Results were presented as mean ± SEM. N = 5, ** P < 0.01, *** P < 0.001, one-way ANOVA followed by Tukey’s post hoc test.

    Journal: Renal Failure

    Article Title: ADAMTS13 ameliorates diabetic nephropathy by Nrf2/GPX4/eNOS signaling pathway

    doi: 10.1080/0886022X.2026.2646089

    Figure Lengend Snippet: ADAMTS13 maintained renal function and attenuated renal fibrosis in vivo . Control mice were treated with vehicle (Control). The STZ-treated mice were infused with vehicle (DN) or rhADAMTS13 (DN + rhADAMTS13). rhADAMTS13 (2.6 ug/kg body weight) was injected into the tail vein daily for the subsequent 7 d. (A) The workflow of animal experiments. (B) Representative renal immunohistochemical staining of ADAMTS13 in the kidney. Scale Bar: 30 μm. (C) Representative renal morphological images of mice were shown. Hematoxylin-Eosin (HE), Masson’s trichrome and Periodic Acid-Schiff (PAS) staining of kidney slices. Scale Bar: 30 μm. (D) Body weight. (E) Serum glucose. (F) BUN. (G) Scr. (H) Urinary volume. (I) Proteinuria. (J) Urinary KIM-1. (K) Renal KIM-1 mRNA expression. ADAMTS13: a disintegrin and metalloprotease with a thrombospondin type 1 motif member 13; DN: diabetic nephropathy; BUN: blood urea nitrogen; Scr: serum creatinine; KIM-1: kidney injury molecule-1. Results were presented as mean ± SEM. N = 5, ** P < 0.01, *** P < 0.001, one-way ANOVA followed by Tukey’s post hoc test.

    Article Snippet: Hyperglycemia was defined as a fasting blood glucose ≥11.1 mmol/L or random blood glucose ≥16.7 mmol/L. rhADAMTS13 (4245-AD, R&D Systems, Minneapolis, MN) was dissolved in saline and administered to mice via tail vein injections.

    Techniques: In Vivo, Control, Injection, Immunohistochemical staining, Staining, Expressing

    ADAMTS13 degradation by purified proteases and in plasma-based assays. (A) Commercial rADAMTS13 (200 nM) was incubated with various proteases (100 nM) for 2 hours at 37°C. Samples were separated under reducing conditions using SDS-PAGE, and cleavage was visualized using SYPRO Ruby. (B) rADAMTS13, varying dilutions of tissue factor (TF), and 1 mg/mL GPRP-amide were incubated in human platelet-poor plasma for 15 minutes. Thrombin generation was then initiated with calcium and quantified over time in a parallel plate by measuring fluorescence (λ ex = 360 nm; λ em = 460) at 37°C. Curves were generated using the Technothrombin TGA Software in triplicate, and representative images are shown. (C) rADAMTS13 and varying concentrations of tPA were added to human platelet-poor plasma, followed by TF and calcium. Clot formation and lysis was quantified by measuring OD at 405 nm every 30 seconds in triplicate, and representative images are shown. Samples were separated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions, and ADAMTS13 degradation was visualized via western blot using an anti-ADAMTS13 metalloprotease domain antibody. Molecular weight references are indicated on the left of blots (kDa), and full-length bands are indicated by the black arrows. APC, activated protein C; GPRP-amide, Gly-Pro-Arg-Pro-amide; OD, optical density.

    Journal: Blood Advances

    Article Title: Development of a protease-resistant ADAMTS13 to improve stability against proteolytic degradation ∗

    doi: 10.1182/bloodadvances.2024015212

    Figure Lengend Snippet: ADAMTS13 degradation by purified proteases and in plasma-based assays. (A) Commercial rADAMTS13 (200 nM) was incubated with various proteases (100 nM) for 2 hours at 37°C. Samples were separated under reducing conditions using SDS-PAGE, and cleavage was visualized using SYPRO Ruby. (B) rADAMTS13, varying dilutions of tissue factor (TF), and 1 mg/mL GPRP-amide were incubated in human platelet-poor plasma for 15 minutes. Thrombin generation was then initiated with calcium and quantified over time in a parallel plate by measuring fluorescence (λ ex = 360 nm; λ em = 460) at 37°C. Curves were generated using the Technothrombin TGA Software in triplicate, and representative images are shown. (C) rADAMTS13 and varying concentrations of tPA were added to human platelet-poor plasma, followed by TF and calcium. Clot formation and lysis was quantified by measuring OD at 405 nm every 30 seconds in triplicate, and representative images are shown. Samples were separated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions, and ADAMTS13 degradation was visualized via western blot using an anti-ADAMTS13 metalloprotease domain antibody. Molecular weight references are indicated on the left of blots (kDa), and full-length bands are indicated by the black arrows. APC, activated protein C; GPRP-amide, Gly-Pro-Arg-Pro-amide; OD, optical density.

    Article Snippet: Recombinant (r)ADAMTS13 and human ADAMTS13 Quantikine enzyme-linked immunosorbent assay kit (R&D Systems, Minneapolis, MN), kallikrein, FXIIa (Enzyme Research Laboratories, South Bend, IN), thrombin, FXa, FIXa, FXIa, FVIIa, activated protein C (APC), elastase, cathepsin G (Sigma-Aldrich, St Louis, MO), proteinase 3 (Enzo Life Sciences, Inc, Farmingdale, NY), FRETS-VWF73 (AnaSpec, Fremont, CA), AEBSF (4-[2-aminoethyl]benzenesulfonyl fluoride hydrochloride), Sivelestat (Tocris Bioscience, Bristol, United Kingdom), and cathepsin G inhibitor 1 (Cayman Chemical Company, Ann Arbor, MI).

    Techniques: Purification, Clinical Proteomics, Incubation, SDS Page, Fluorescence, Generated, Software, Lysis, Polyacrylamide Gel Electrophoresis, Western Blot, Molecular Weight

    ADAMTS13 degradation by activated neutrophils. (A) ADAMTS13 was incubated with 0 × 10 3 to 500 × 10 3 PMA-stimulated neutrophils for 1 hour at 37°C, in the absence or presence of 2 mM AEBSF or 5 mM EDTA (B). (C) ADAMTS13 was incubated with 5 × 10 3 PMA-stimulated neutrophils with increasing concentrations of AEBSF, cathepsinG inhibitor I (CGI I), or Sivelestat for 1 hour. Samples were separated via SDS-PAGE and analyzed by (A) western blot using an anti-ADAMTS13 metalloprotease domain antibody, or (B-C) SYPRO Ruby. Black arrows indicate the level of full-length ADAMTS13 (∼180 kDa). AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride; EDTA, ethylenediaminetetraacetic acid.

    Journal: Blood Advances

    Article Title: Development of a protease-resistant ADAMTS13 to improve stability against proteolytic degradation ∗

    doi: 10.1182/bloodadvances.2024015212

    Figure Lengend Snippet: ADAMTS13 degradation by activated neutrophils. (A) ADAMTS13 was incubated with 0 × 10 3 to 500 × 10 3 PMA-stimulated neutrophils for 1 hour at 37°C, in the absence or presence of 2 mM AEBSF or 5 mM EDTA (B). (C) ADAMTS13 was incubated with 5 × 10 3 PMA-stimulated neutrophils with increasing concentrations of AEBSF, cathepsinG inhibitor I (CGI I), or Sivelestat for 1 hour. Samples were separated via SDS-PAGE and analyzed by (A) western blot using an anti-ADAMTS13 metalloprotease domain antibody, or (B-C) SYPRO Ruby. Black arrows indicate the level of full-length ADAMTS13 (∼180 kDa). AEBSF, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride; EDTA, ethylenediaminetetraacetic acid.

    Article Snippet: Recombinant (r)ADAMTS13 and human ADAMTS13 Quantikine enzyme-linked immunosorbent assay kit (R&D Systems, Minneapolis, MN), kallikrein, FXIIa (Enzyme Research Laboratories, South Bend, IN), thrombin, FXa, FIXa, FXIa, FVIIa, activated protein C (APC), elastase, cathepsin G (Sigma-Aldrich, St Louis, MO), proteinase 3 (Enzo Life Sciences, Inc, Farmingdale, NY), FRETS-VWF73 (AnaSpec, Fremont, CA), AEBSF (4-[2-aminoethyl]benzenesulfonyl fluoride hydrochloride), Sivelestat (Tocris Bioscience, Bristol, United Kingdom), and cathepsin G inhibitor 1 (Cayman Chemical Company, Ann Arbor, MI).

    Techniques: Incubation, SDS Page, Western Blot

    Characterizing the resistance of the ADAMTS13 mutants against proteolysis by purified proteases and in plasma fibrinolysis. (A) Four mutants of ADAMTS13 were developed: 2 with 1 of each C-terminal linker region mutated (T4L and T8L mutants), 1 with both linker regions mutated (T4L/T8L mutant), and a fourth with both linker regions and an additional elastase cleavage site mutation (T4L/T8L[I380G] mutant). (B) Using commercial recombinant ADAMTS13 (WT) as a control, each linker mutant (T4L, T8L, and/or T4L/T8L) was incubated at 50 nM with purified coagulation and fibrinolytic proteases at 50 nM (plasmin, thrombin, and kallikrein) or 100 nM (FXIa) for 0 to 180 minutes at 37°C. (C) The T4L/T8L mutant and 10 nM tPA were added to human platelet-poor plasma, followed by tissue factor and calcium at 37°C. Aliquots were removed at 0 and 45 minutes. (D) Mutants were incubated with 50 nM purified neutrophil-derived proteases (elastase, proteinase 3, and cathepsin G) at 37°C. The T4L/T8L(I380G) mutant was also incubated with 50 nM elastase. Western blotting was performed using an anti-ADAMTS13 metalloprotease domain antibody. The band corresponding to full-length protein (∼180 kDa) is indicated by black arrows.

    Journal: Blood Advances

    Article Title: Development of a protease-resistant ADAMTS13 to improve stability against proteolytic degradation ∗

    doi: 10.1182/bloodadvances.2024015212

    Figure Lengend Snippet: Characterizing the resistance of the ADAMTS13 mutants against proteolysis by purified proteases and in plasma fibrinolysis. (A) Four mutants of ADAMTS13 were developed: 2 with 1 of each C-terminal linker region mutated (T4L and T8L mutants), 1 with both linker regions mutated (T4L/T8L mutant), and a fourth with both linker regions and an additional elastase cleavage site mutation (T4L/T8L[I380G] mutant). (B) Using commercial recombinant ADAMTS13 (WT) as a control, each linker mutant (T4L, T8L, and/or T4L/T8L) was incubated at 50 nM with purified coagulation and fibrinolytic proteases at 50 nM (plasmin, thrombin, and kallikrein) or 100 nM (FXIa) for 0 to 180 minutes at 37°C. (C) The T4L/T8L mutant and 10 nM tPA were added to human platelet-poor plasma, followed by tissue factor and calcium at 37°C. Aliquots were removed at 0 and 45 minutes. (D) Mutants were incubated with 50 nM purified neutrophil-derived proteases (elastase, proteinase 3, and cathepsin G) at 37°C. The T4L/T8L(I380G) mutant was also incubated with 50 nM elastase. Western blotting was performed using an anti-ADAMTS13 metalloprotease domain antibody. The band corresponding to full-length protein (∼180 kDa) is indicated by black arrows.

    Article Snippet: Recombinant (r)ADAMTS13 and human ADAMTS13 Quantikine enzyme-linked immunosorbent assay kit (R&D Systems, Minneapolis, MN), kallikrein, FXIIa (Enzyme Research Laboratories, South Bend, IN), thrombin, FXa, FIXa, FXIa, FVIIa, activated protein C (APC), elastase, cathepsin G (Sigma-Aldrich, St Louis, MO), proteinase 3 (Enzo Life Sciences, Inc, Farmingdale, NY), FRETS-VWF73 (AnaSpec, Fremont, CA), AEBSF (4-[2-aminoethyl]benzenesulfonyl fluoride hydrochloride), Sivelestat (Tocris Bioscience, Bristol, United Kingdom), and cathepsin G inhibitor 1 (Cayman Chemical Company, Ann Arbor, MI).

    Techniques: Purification, Clinical Proteomics, Mutagenesis, Recombinant, Control, Incubation, Coagulation, Derivative Assay, Western Blot

    Resistance of ADAMTS13 mutants to activated neutrophils. 50 nM WT-, T4L/T8L-, or T4L/T8L(I380G)-ADAMTS13 was incubated with 0 × 10 3 to 50 × 10 3 PMA-activated neutrophils, or 500 × 10 3 neutrophils, as indicated. Reactions took place for 1 hour at 37°C, then samples were removed and separated via SDS-PAGE under reducing conditions. Cleavage was visualized by western blot using an anti-ADAMTS13 metalloprotease domain antibody. Molecular weight references are indicated to the left (kDa). The level of full-length protein (∼180 kDa) is indicated by black arrows.

    Journal: Blood Advances

    Article Title: Development of a protease-resistant ADAMTS13 to improve stability against proteolytic degradation ∗

    doi: 10.1182/bloodadvances.2024015212

    Figure Lengend Snippet: Resistance of ADAMTS13 mutants to activated neutrophils. 50 nM WT-, T4L/T8L-, or T4L/T8L(I380G)-ADAMTS13 was incubated with 0 × 10 3 to 50 × 10 3 PMA-activated neutrophils, or 500 × 10 3 neutrophils, as indicated. Reactions took place for 1 hour at 37°C, then samples were removed and separated via SDS-PAGE under reducing conditions. Cleavage was visualized by western blot using an anti-ADAMTS13 metalloprotease domain antibody. Molecular weight references are indicated to the left (kDa). The level of full-length protein (∼180 kDa) is indicated by black arrows.

    Article Snippet: Recombinant (r)ADAMTS13 and human ADAMTS13 Quantikine enzyme-linked immunosorbent assay kit (R&D Systems, Minneapolis, MN), kallikrein, FXIIa (Enzyme Research Laboratories, South Bend, IN), thrombin, FXa, FIXa, FXIa, FVIIa, activated protein C (APC), elastase, cathepsin G (Sigma-Aldrich, St Louis, MO), proteinase 3 (Enzo Life Sciences, Inc, Farmingdale, NY), FRETS-VWF73 (AnaSpec, Fremont, CA), AEBSF (4-[2-aminoethyl]benzenesulfonyl fluoride hydrochloride), Sivelestat (Tocris Bioscience, Bristol, United Kingdom), and cathepsin G inhibitor 1 (Cayman Chemical Company, Ann Arbor, MI).

    Techniques: Incubation, SDS Page, Western Blot, Molecular Weight

    Activity of ADAMTS13, MDTCS, and T4L/T8L-ADAMTS13 in a microfluidic flow assay. DiOC6-stained platelets were perfused over a microfluidic flow chamber lined with histamine-stimulated HUVECs, forming stable VWF-platelet strings. Channels were then perfused with 5 mL of 5 nM MDTCS, 5 nM WT-ADAMTS13, 5 nM T4L/T8L-ADAMTS13, or ADAMTS13 reaction buffer (n = 3). Using a Leica Stellaris 5 inverted confocal microscope (10× dry lens; λ ex = 490 nm; λ em = 525 nm), videos were captured at 0.77 frames per second; (A) photographic representation of experimental replicate. Experiments occurred at room temperature. The total length of strings within the frame was quantified every 20 frames for 5 frames. Rates were determined using linear regression (B), and differences between rates were assessed using unpaired t tests . Error bars represent standard deviation, and symbols represent 4 experimental conditions: buffer (▲), MDTCS (■), WT-ADAMTS13 (●), T4L/T8L-ADAMTS13 (▼). ∗P < .05, vs MDTCS.

    Journal: Blood Advances

    Article Title: Development of a protease-resistant ADAMTS13 to improve stability against proteolytic degradation ∗

    doi: 10.1182/bloodadvances.2024015212

    Figure Lengend Snippet: Activity of ADAMTS13, MDTCS, and T4L/T8L-ADAMTS13 in a microfluidic flow assay. DiOC6-stained platelets were perfused over a microfluidic flow chamber lined with histamine-stimulated HUVECs, forming stable VWF-platelet strings. Channels were then perfused with 5 mL of 5 nM MDTCS, 5 nM WT-ADAMTS13, 5 nM T4L/T8L-ADAMTS13, or ADAMTS13 reaction buffer (n = 3). Using a Leica Stellaris 5 inverted confocal microscope (10× dry lens; λ ex = 490 nm; λ em = 525 nm), videos were captured at 0.77 frames per second; (A) photographic representation of experimental replicate. Experiments occurred at room temperature. The total length of strings within the frame was quantified every 20 frames for 5 frames. Rates were determined using linear regression (B), and differences between rates were assessed using unpaired t tests . Error bars represent standard deviation, and symbols represent 4 experimental conditions: buffer (▲), MDTCS (■), WT-ADAMTS13 (●), T4L/T8L-ADAMTS13 (▼). ∗P < .05, vs MDTCS.

    Article Snippet: Recombinant (r)ADAMTS13 and human ADAMTS13 Quantikine enzyme-linked immunosorbent assay kit (R&D Systems, Minneapolis, MN), kallikrein, FXIIa (Enzyme Research Laboratories, South Bend, IN), thrombin, FXa, FIXa, FXIa, FVIIa, activated protein C (APC), elastase, cathepsin G (Sigma-Aldrich, St Louis, MO), proteinase 3 (Enzo Life Sciences, Inc, Farmingdale, NY), FRETS-VWF73 (AnaSpec, Fremont, CA), AEBSF (4-[2-aminoethyl]benzenesulfonyl fluoride hydrochloride), Sivelestat (Tocris Bioscience, Bristol, United Kingdom), and cathepsin G inhibitor 1 (Cayman Chemical Company, Ann Arbor, MI).

    Techniques: Activity Assay, Staining, Microscopy, Standard Deviation

    Fig. 3. Effect of rhADAMTS13 on plasma ADAMTS13 activity and vWF-Ag balance and coagulation function in AKI-F mice. (A) Experimental design of treatment with rhADAMTS13 against AKI-F mice. (B) Body weight at the end of experiment. (C and D) Plasma ADAMTS13 activity (C) and plasma vWF-Ag level (D). The values are indicated as % NC group. (E) Pearson’s correlation between plasma ADAMTS13 activity and vWF-Ag level in all of experimental mice. (F) Multimer distribution and the ratios of high (H) to low (L) molecular weight of vWF multimers. (G and H) Platelet count and plasma D-dimer level. Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; B − D, G and H) or Mann−Whitney U test (n = 3; F). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis.

    Journal: Biochimica et biophysica acta. Molecular cell research

    Article Title: Recombinant human ADAMTS13 attenuates LPS-induced acute kidney injury and renal microangiopathy in murine advanced liver fibrosis by cleaving vWF.

    doi: 10.1016/j.bbamcr.2025.120000

    Figure Lengend Snippet: Fig. 3. Effect of rhADAMTS13 on plasma ADAMTS13 activity and vWF-Ag balance and coagulation function in AKI-F mice. (A) Experimental design of treatment with rhADAMTS13 against AKI-F mice. (B) Body weight at the end of experiment. (C and D) Plasma ADAMTS13 activity (C) and plasma vWF-Ag level (D). The values are indicated as % NC group. (E) Pearson’s correlation between plasma ADAMTS13 activity and vWF-Ag level in all of experimental mice. (F) Multimer distribution and the ratios of high (H) to low (L) molecular weight of vWF multimers. (G and H) Platelet count and plasma D-dimer level. Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; B − D, G and H) or Mann−Whitney U test (n = 3; F). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis.

    Article Snippet: Recombinant Human ADAMTS13 (Full Length) Protein, Carrier-free used as rhADAMTS13 was obtained from R&D systems (Minneapolis, MN, USA).

    Techniques: Clinical Proteomics, Activity Assay, Coagulation, Molecular Weight, MANN-WHITNEY, Control

    Fig. 6. Effect of rADAMTS13 on renal microthrombus and microangiopathy in AKI-F mice. (A) Renal ADAMTS13 (A) and vWF (B) levels. The values are indicated as % NC group. (C) Pearson’s correlation between renal ADAMTS13 and vWF levels in all of experimental mice. (D) Representative photographs of CD41a staining in kidney tissue of NC, D + 2, D + 3 (with and without treatment with rhADAMTS13) groups. (E) Quantification of CD41a+ microthrombus in kidney tissues. DAPI was used as nuclear staining. (F) Microvascular blood flow in the bilateral kidney tissues. (G) Renal mRNA levels of angiogenic factors (Hif1a, Vegfa, Vegfr2, Angpt1, and Tie2). (H) Renal mRNA levels of vascular inflammation markers (Vcam1, Icam1, Sele, and Selp). Gapdh was used as an internal control for qRT-PCR (G and H). The values are indicated as the ratio to NC group (E − H). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; A, B, F −H, n = 6; E). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13.

    Journal: Biochimica et biophysica acta. Molecular cell research

    Article Title: Recombinant human ADAMTS13 attenuates LPS-induced acute kidney injury and renal microangiopathy in murine advanced liver fibrosis by cleaving vWF.

    doi: 10.1016/j.bbamcr.2025.120000

    Figure Lengend Snippet: Fig. 6. Effect of rADAMTS13 on renal microthrombus and microangiopathy in AKI-F mice. (A) Renal ADAMTS13 (A) and vWF (B) levels. The values are indicated as % NC group. (C) Pearson’s correlation between renal ADAMTS13 and vWF levels in all of experimental mice. (D) Representative photographs of CD41a staining in kidney tissue of NC, D + 2, D + 3 (with and without treatment with rhADAMTS13) groups. (E) Quantification of CD41a+ microthrombus in kidney tissues. DAPI was used as nuclear staining. (F) Microvascular blood flow in the bilateral kidney tissues. (G) Renal mRNA levels of angiogenic factors (Hif1a, Vegfa, Vegfr2, Angpt1, and Tie2). (H) Renal mRNA levels of vascular inflammation markers (Vcam1, Icam1, Sele, and Selp). Gapdh was used as an internal control for qRT-PCR (G and H). The values are indicated as the ratio to NC group (E − H). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; A, B, F −H, n = 6; E). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13.

    Article Snippet: Recombinant Human ADAMTS13 (Full Length) Protein, Carrier-free used as rhADAMTS13 was obtained from R&D systems (Minneapolis, MN, USA).

    Techniques: Staining, Control, Quantitative RT-PCR, Recombinant

    Fig. 3. Effect of rhADAMTS13 on plasma ADAMTS13 activity and vWF-Ag balance and coagulation function in AKI-F mice. (A) Experimental design of treatment with rhADAMTS13 against AKI-F mice. (B) Body weight at the end of experiment. (C and D) Plasma ADAMTS13 activity (C) and plasma vWF-Ag level (D). The values are indicated as % NC group. (E) Pearson’s correlation between plasma ADAMTS13 activity and vWF-Ag level in all of experimental mice. (F) Multimer distribution and the ratios of high (H) to low (L) molecular weight of vWF multimers. (G and H) Platelet count and plasma D-dimer level. Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; B − D, G and H) or Mann−Whitney U test (n = 3; F). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis.

    Journal: Biochimica et biophysica acta. Molecular cell research

    Article Title: Recombinant human ADAMTS13 attenuates LPS-induced acute kidney injury and renal microangiopathy in murine advanced liver fibrosis by cleaving vWF.

    doi: 10.1016/j.bbamcr.2025.120000

    Figure Lengend Snippet: Fig. 3. Effect of rhADAMTS13 on plasma ADAMTS13 activity and vWF-Ag balance and coagulation function in AKI-F mice. (A) Experimental design of treatment with rhADAMTS13 against AKI-F mice. (B) Body weight at the end of experiment. (C and D) Plasma ADAMTS13 activity (C) and plasma vWF-Ag level (D). The values are indicated as % NC group. (E) Pearson’s correlation between plasma ADAMTS13 activity and vWF-Ag level in all of experimental mice. (F) Multimer distribution and the ratios of high (H) to low (L) molecular weight of vWF multimers. (G and H) Platelet count and plasma D-dimer level. Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; B − D, G and H) or Mann−Whitney U test (n = 3; F). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis.

    Article Snippet: Recombinant Human ADAMTS13 (Full Length) Protein, Carrier-free used as rhADAMTS13 was obtained from R&D systems (Minneapolis, MN, USA).

    Techniques: Clinical Proteomics, Activity Assay, Coagulation, Molecular Weight, MANN-WHITNEY, Control

    Fig. 4. Effect of rADAMTS13 on liver damage and hepatic ischemia in in AKI-F mice. (A and B) Hepatic ADAMTS13 (A) and vWF (B) levels. The values are indicated as % NC group. (C) Pearson’s correlation between hepatic ADAMTS13 and vWF levels in all of experimental mice. (D) Microvascular blood flow in the liver tissue. The values are indicated as the ratio to control. (E and F) Serum levels of transaminases and total bilirubin (T-bil) (E) and albumin (Alb) (F) at the end of experiment. (H) Representative photographs of H&E and Sirius-Red staining in liver tissue. (G) Ratio of liver to body weight at the end of experiment. (I) Quantification of necrotic regions in liver tissue based on H&E staining. The values are indicated as % necrotic area in high power field. (J) Quantification of fibrotic area in liver tissue based on Sirius-Red staining. The values are indicated as the ratio to control. Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; A, B, D-G, I, and J). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13; AST, aspartate transaminase; ALT, alanine aminotransferase. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Biochimica et biophysica acta. Molecular cell research

    Article Title: Recombinant human ADAMTS13 attenuates LPS-induced acute kidney injury and renal microangiopathy in murine advanced liver fibrosis by cleaving vWF.

    doi: 10.1016/j.bbamcr.2025.120000

    Figure Lengend Snippet: Fig. 4. Effect of rADAMTS13 on liver damage and hepatic ischemia in in AKI-F mice. (A and B) Hepatic ADAMTS13 (A) and vWF (B) levels. The values are indicated as % NC group. (C) Pearson’s correlation between hepatic ADAMTS13 and vWF levels in all of experimental mice. (D) Microvascular blood flow in the liver tissue. The values are indicated as the ratio to control. (E and F) Serum levels of transaminases and total bilirubin (T-bil) (E) and albumin (Alb) (F) at the end of experiment. (H) Representative photographs of H&E and Sirius-Red staining in liver tissue. (G) Ratio of liver to body weight at the end of experiment. (I) Quantification of necrotic regions in liver tissue based on H&E staining. The values are indicated as % necrotic area in high power field. (J) Quantification of fibrotic area in liver tissue based on Sirius-Red staining. The values are indicated as the ratio to control. Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; A, B, D-G, I, and J). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13; AST, aspartate transaminase; ALT, alanine aminotransferase. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Recombinant Human ADAMTS13 (Full Length) Protein, Carrier-free used as rhADAMTS13 was obtained from R&D systems (Minneapolis, MN, USA).

    Techniques: Control, Staining, Recombinant

    Fig. 5. Effect of rADAMTS13 on hepatic macrophage infiltration and oxidative damage in AKI-F mice. (A) Representative photographs of F4/80 staining in liver tissue. (B) Quantification of F4/80-positive macrophage in liver tissue. (C) Hepatic mRNA levels of proinflammatory cytokines (Tnfa, Il1b and Il6). (D) Representative photographs of 4-hydroxynonenal (4-HNE) staining in liver tissue. (E) Quantification of 4-HNE-positive area in liver tissue. (F) Hepatic mRNA levels of NADPH oxidases (Nox1, Nox2 and Nox4). DAPI was used as nuclear staining (A and D). Gapdh was used as an internal control for qRT-PCR (C and F). The values are indicated as the ratio to NC group (B, C, E, and F). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; B, C, E, and F). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13.

    Journal: Biochimica et biophysica acta. Molecular cell research

    Article Title: Recombinant human ADAMTS13 attenuates LPS-induced acute kidney injury and renal microangiopathy in murine advanced liver fibrosis by cleaving vWF.

    doi: 10.1016/j.bbamcr.2025.120000

    Figure Lengend Snippet: Fig. 5. Effect of rADAMTS13 on hepatic macrophage infiltration and oxidative damage in AKI-F mice. (A) Representative photographs of F4/80 staining in liver tissue. (B) Quantification of F4/80-positive macrophage in liver tissue. (C) Hepatic mRNA levels of proinflammatory cytokines (Tnfa, Il1b and Il6). (D) Representative photographs of 4-hydroxynonenal (4-HNE) staining in liver tissue. (E) Quantification of 4-HNE-positive area in liver tissue. (F) Hepatic mRNA levels of NADPH oxidases (Nox1, Nox2 and Nox4). DAPI was used as nuclear staining (A and D). Gapdh was used as an internal control for qRT-PCR (C and F). The values are indicated as the ratio to NC group (B, C, E, and F). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; B, C, E, and F). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13.

    Article Snippet: Recombinant Human ADAMTS13 (Full Length) Protein, Carrier-free used as rhADAMTS13 was obtained from R&D systems (Minneapolis, MN, USA).

    Techniques: Staining, Control, Quantitative RT-PCR, Recombinant

    Fig. 6. Effect of rADAMTS13 on renal microthrombus and microangiopathy in AKI-F mice. (A) Renal ADAMTS13 (A) and vWF (B) levels. The values are indicated as % NC group. (C) Pearson’s correlation between renal ADAMTS13 and vWF levels in all of experimental mice. (D) Representative photographs of CD41a staining in kidney tissue of NC, D + 2, D + 3 (with and without treatment with rhADAMTS13) groups. (E) Quantification of CD41a+ microthrombus in kidney tissues. DAPI was used as nuclear staining. (F) Microvascular blood flow in the bilateral kidney tissues. (G) Renal mRNA levels of angiogenic factors (Hif1a, Vegfa, Vegfr2, Angpt1, and Tie2). (H) Renal mRNA levels of vascular inflammation markers (Vcam1, Icam1, Sele, and Selp). Gapdh was used as an internal control for qRT-PCR (G and H). The values are indicated as the ratio to NC group (E − H). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; A, B, F −H, n = 6; E). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13.

    Journal: Biochimica et biophysica acta. Molecular cell research

    Article Title: Recombinant human ADAMTS13 attenuates LPS-induced acute kidney injury and renal microangiopathy in murine advanced liver fibrosis by cleaving vWF.

    doi: 10.1016/j.bbamcr.2025.120000

    Figure Lengend Snippet: Fig. 6. Effect of rADAMTS13 on renal microthrombus and microangiopathy in AKI-F mice. (A) Renal ADAMTS13 (A) and vWF (B) levels. The values are indicated as % NC group. (C) Pearson’s correlation between renal ADAMTS13 and vWF levels in all of experimental mice. (D) Representative photographs of CD41a staining in kidney tissue of NC, D + 2, D + 3 (with and without treatment with rhADAMTS13) groups. (E) Quantification of CD41a+ microthrombus in kidney tissues. DAPI was used as nuclear staining. (F) Microvascular blood flow in the bilateral kidney tissues. (G) Renal mRNA levels of angiogenic factors (Hif1a, Vegfa, Vegfr2, Angpt1, and Tie2). (H) Renal mRNA levels of vascular inflammation markers (Vcam1, Icam1, Sele, and Selp). Gapdh was used as an internal control for qRT-PCR (G and H). The values are indicated as the ratio to NC group (E − H). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; A, B, F −H, n = 6; E). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13.

    Article Snippet: Recombinant Human ADAMTS13 (Full Length) Protein, Carrier-free used as rhADAMTS13 was obtained from R&D systems (Minneapolis, MN, USA).

    Techniques: Staining, Control, Quantitative RT-PCR, Recombinant

    Fig. 7. Effect of rADAMTS13 on kidney damage in cirrhotic mice with AKI. (A-E) Serum level of kidney injury markers including BUN (A), Scr (B), KIM-1 (C), osteopontin (OPN) (D), and neutrophil gelatinase-associated lipocalin (NGAL) (E). (F) Ratio of bilateral kidneys to body weight at the end of experiment. (G) Representative photographs of H&E, PAS and KIM-1 staining in kidney tissue. (H) Kidney injury score based on both H&E and PAS staining. (I) Quantification of KIM- 1-positive area in kidney tissue. (J) Renal mRNA levels of kidney injury markers (Havcr1 and Vim). Gapdh was used as an internal control for qRT-PCR (J). The values are indicated as the ratio to NC group (F, I, and J). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t- test (n = 9; A-F and H-J). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombi nant ADAMTS13.

    Journal: Biochimica et biophysica acta. Molecular cell research

    Article Title: Recombinant human ADAMTS13 attenuates LPS-induced acute kidney injury and renal microangiopathy in murine advanced liver fibrosis by cleaving vWF.

    doi: 10.1016/j.bbamcr.2025.120000

    Figure Lengend Snippet: Fig. 7. Effect of rADAMTS13 on kidney damage in cirrhotic mice with AKI. (A-E) Serum level of kidney injury markers including BUN (A), Scr (B), KIM-1 (C), osteopontin (OPN) (D), and neutrophil gelatinase-associated lipocalin (NGAL) (E). (F) Ratio of bilateral kidneys to body weight at the end of experiment. (G) Representative photographs of H&E, PAS and KIM-1 staining in kidney tissue. (H) Kidney injury score based on both H&E and PAS staining. (I) Quantification of KIM- 1-positive area in kidney tissue. (J) Renal mRNA levels of kidney injury markers (Havcr1 and Vim). Gapdh was used as an internal control for qRT-PCR (J). The values are indicated as the ratio to NC group (F, I, and J). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t- test (n = 9; A-F and H-J). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombi nant ADAMTS13.

    Article Snippet: Recombinant Human ADAMTS13 (Full Length) Protein, Carrier-free used as rhADAMTS13 was obtained from R&D systems (Minneapolis, MN, USA).

    Techniques: Staining, Control, Quantitative RT-PCR

    Fig. 8. Effect of rADAMTS13 on renal macrophage infiltration, oxidative damage and fibrosis in cirrhotic mice with AKI. (A) Representative photographs of F4/80 and 4-HNE staining in kidney tissue. (B and C) Quantification of F4/80 (B) and 4-HNE (C)-positive area in kidney tissue. (D) Renal mRNA levels of proinflammatory cytokines (Tnfa, Il1b and Il6). (E-G) Renal mRNA levels of NADPH oxidases (Nox2 and Nox4) (E), antioxidant enzymes (Sod2 and Cat) (F) and Hmox1 (G). (H) Representative photographs of Sirius-Red staining in kidney tissue. (I) Quantification of fibrotic area in kidney tissue based on Sirius-Red staining. (J) Renal mRNA levels of fibrogenic markers (Col1a1, Acta2, and Tgfb1). DAPI was used as nuclear staining (A). Gapdh was used as an internal control for qRT-PCR (D, E and J). The values are indicated as the ratio to NC group (B-G, I, and J). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; B-G, I, and J). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Biochimica et biophysica acta. Molecular cell research

    Article Title: Recombinant human ADAMTS13 attenuates LPS-induced acute kidney injury and renal microangiopathy in murine advanced liver fibrosis by cleaving vWF.

    doi: 10.1016/j.bbamcr.2025.120000

    Figure Lengend Snippet: Fig. 8. Effect of rADAMTS13 on renal macrophage infiltration, oxidative damage and fibrosis in cirrhotic mice with AKI. (A) Representative photographs of F4/80 and 4-HNE staining in kidney tissue. (B and C) Quantification of F4/80 (B) and 4-HNE (C)-positive area in kidney tissue. (D) Renal mRNA levels of proinflammatory cytokines (Tnfa, Il1b and Il6). (E-G) Renal mRNA levels of NADPH oxidases (Nox2 and Nox4) (E), antioxidant enzymes (Sod2 and Cat) (F) and Hmox1 (G). (H) Representative photographs of Sirius-Red staining in kidney tissue. (I) Quantification of fibrotic area in kidney tissue based on Sirius-Red staining. (J) Renal mRNA levels of fibrogenic markers (Col1a1, Acta2, and Tgfb1). DAPI was used as nuclear staining (A). Gapdh was used as an internal control for qRT-PCR (D, E and J). The values are indicated as the ratio to NC group (B-G, I, and J). Data are the mean ± SD, and *: p < 0.05, **: p < 0.01 with significant difference between groups by Student’s t-test (n = 9; B-G, I, and J). NC, normal control without CCl4 and LPS administration; AKI-F, acute kidney injury mice with advanced liver fibrosis; rAD, recombinant ADAMTS13. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Recombinant Human ADAMTS13 (Full Length) Protein, Carrier-free used as rhADAMTS13 was obtained from R&D systems (Minneapolis, MN, USA).

    Techniques: Staining, Control, Quantitative RT-PCR, Recombinant